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기기명(국문) 디지털 도립 형광 현미경 (Nikon) 디지털 도립 형광 현미경 (Nikon)의 사진
기기명(영문) Digital Inverted Fluorescence Microscope (Nikon)
제조사 Nikon/Japan
모델명 DS-Ri2 / Nikon Ti
설치장소 본관 210호
담당자 박향림
담당자연락처 02-740-8708
용도 및 원리 조직 및 배양세포의 광학 및 형광 이미지 관찰 및 디지털 영상 저장
camera :
This camera is designed to provide low light qualitative and quantitative fluorescence image capture together in one unit.
Applications range from high resolution color brightfield to low light monochrome quantitative image capture.
원리
시료 자체가 형광성을 가지고 있고, 형광물질을 흡착할 수 있는 것에 유효. 자외선 발생장치와 광원필터를 사용하여 암실 또는 반암실 내에서 보통의 광학현미경에 의해 관찰하며, 개개의 시료에 따라서 적합한 형광색소를 선택한다.
극미량의 미세한 형광 변화와, 형광 이미지 관찰
주요성능 ■ Ti (Nikon) : Brightfield, Fluorescence 관찰용 inverted microscope
- 3 Kinds of filter block for UV,B and G they comprese and exiter filter, a dichroic mirron, and barrier filter.
1) Ultra-violet filter range : Excitation filter EX340-380 dichronic mirror DM400 and barrier filter, BA 420
2) Blue filter range : Excitation filter EX450-490 dichronic mirror DM505, and barrier filter BA520
3) Green filter range : Excitation filter EX510/560 dichronic mirror DM575, and barrier filter BA590

-Objectives:
1) CFIPlanFluorDL4XF N.A. 0.13, W.D. 16.4 mm, PhL
2) CFIPlanFluorDL10XF N.A. 0.30, W.D. 15.2 mm, Ph1
3) CFISuperPlanFluorELWDADM20XC N.A. 0.45, W.D. 8.2-6.9mm, PH-1
4) CFISuperPlanFluorELWDADM40XC N.A. 0.60, W.D. 3.6-2.8mm, PH-2

■ DS-Ri2: Camera (Nikon)
- Color Cooled Digital Camera Head
1) High-resolution images: 16.25-megapixel CMOS sensors
: images with resolution as high as 4908 x 3264 pixels, without pixel shifting of pixel stepping.
2) Photography with the natural colors seen through the microscope
3) High-speed live display
: The DS-Ri2 can display 4908x3264 pixel (full-pixel) images at 6 fps, or 1636x1088 pixel (3x3 pixel averaging) images at 45 fps.
4) High sensitivity, low noise
: Sensitivity settings that span the range from ISO200 to ISO12800 allow the capture of vivid fluorescent color images.

■ MetaVue Software
- Aqcuire : Auto expose, Timelapse, Stream
- Displays, loads and saves binary, 8-bit, 16-bit, 24-bit and 48-bit (RGB) image types
- Shading correction and background subtraction
- Overlay multi-fluorescent images with background DIC/Phase Contrast
- Regions of interest include: rectangular, elliptical, line, angle, and free form
- Stack processing : Stack arithmetic, best focus, montage, topographical surface, view orthogonal planes, create movie